Detection of Chlamydia trachomatis in rapidly produced McCoy cell monolayers.
نویسندگان
چکیده
منابع مشابه
Detection of Chlamydia trachomatis in rapidly produced McCoy cell monolayers.
The 24-hour delay between seeding coverslips with cells and inoculating samples for culture of chlamydiae was reduced to less than 1 hour by using coverslips which had been pre-treated with glutaraldehyde-activated gamma-aminopropyl-triethoxysilane. Treated coverslips were not toxic for McCoy cells and even one year after treatment monolayers formed rapidly on them. Furthermore, all of 13 Chlam...
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We compared two methods for identification of Chlamydia trachomatis inclusions in McCoy cell monolayers: conventional iodine staining and immunofluorescence staining with monoclonal antibodies against the species-specific major outer membrane protein antigen of C. trachomatis. Among 878 urethral and cervical specimens tested in parallel, the immunofluorescence method detected eightfold more inc...
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McCoy cells treated in six different ways, in addition to untreated cells, were compared to determine which gave rise to the largest number of Chlamydia trachomatis inclusions when tested with a laboratory-passaged strain. The same batch of cells was treated by irradiation, preinoculation exposure to cytochalasin B or 5-iodo-2'-deoxyuridine, and postinoculation exposure to cycloheximide, hydroc...
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Resveratrol (RESV), an antifungal compound from grapes and other plants, has a distinct ability to inhibit the Chlamydia (C.) trachomatis developmental cycle in McCoy cells, a classic cell line used for chlamydial research. Inoculation of C. trachomatis with increasing amounts of RESV (from 12.5 to 100 μM) gave a dose-dependent reduction in the number of infected McCoy cells visualized by using...
متن کاملCultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.
An isolation technique for Chlamydia trachomatis using McCoy cells is described. In contrast to earlier techniques employing such cells, no pretreatment of the cells was used. The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metaboli...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1980
ISSN: 0021-9746
DOI: 10.1136/jcp.33.6.591